机电学院海聚工程教授报告--Target Cell Detection via Microfluidic Magnetic Beads Assay
作者:机械设计及理论系      时间:2017-05-24

报告题目:Target Cell Detection via Microfluidic Magnetic Beads Assay

报告人:Jiang ZheDepartment of Mechanical EngineeringUniversity of Akron

时间:201752614:00-15:30

地点:基础楼301

报告摘要:

Cell characterization plays an important role in cell biology to monitor environmental conditions, diagnose diseases, and study cell growth and differentiation. Traditional cell characterization methods such as FACS (Fluorescence-activated cell sorting) and SPR (Surface plasmon resonance) assays have been widely used for cell properties measurement. Although these methods provide abundant information of cells, they require accurately labeling cells with fluorescence or radioactive tags, which need lengthy sample preparation and skilled personnel. In addition, the instruments based on these methods are bulky, expensive and typically cannot perform onsite cell analysis.

To overcome these limitations, I present a novel integrated microfluidics device for cell analysis that can not only detect and analyze target cells via a straightforward magnetic beads assay, but also precisely measure cell size distribution and concentration. The microfluidic device consists of two identical micro Coulter counter separated by a fluidic chamber. By passing through the two stage device, target cells, which are magnetically activated by specific binding with the superparamagnetic nanoparticles, can be distinguished from non-target cells via their transit time difference between the 1st and 2nd counter. The transit time difference of each cell can be measured by the micro Coulter counting technology.  Thus from the transit time change we can identify specific cell type and quantify target cell percentage within a mixture cell population. To validate the detection method, target human umbilical vein endothelial cells (HUVECs), which were specifically bounded with CD31+ MACS® magnetic beads, were mixed with non-target cells at various cell percentages (2%, 5%, 10%, 50%, 75% and 100%), and were tested in the device. Test results from the microfluidics device showed that for pure target cells, the average transit time at the two counters were 3.69 ms and 4.38 ms (a 18.7% difference), indicating each target cell can be identified by the device. In addition, the target cell concentrations can be precisely measured from the transit time changes.  We also confirmed that after detection most cells were kept viable and can be collected for further downstream applications. With easy sample preparation and simply device structure, this method is expected to facilitate drug screening, target cell identification and stem cell population analysis.